Characterization of Carica pubescens in Dieng Plateau, Central Java based on morphological characters, antioxidant capacity, and protein banding pattern

AINUN NIKMATI LAILY, SURANTO, SUGIYARTO

 Abstract. Laily AN, Suranto, Sugiyarto. 2012. Characterization of Carica pubescens in Dieng Plateau, Central Java based on morphological characters, antioxidant capacity, and protein banding pattern. Nusantara Bioscience 4: 16-21. Carica pubescens Lenne & K. Koch is a species of fruit plant firstly cultivated in South America and has adapted to the highland environment, such as Dieng Plateau, Central Java (~2000 m asl). It has a narrow habitat range and limited or unknown intraspecies variation. Therefore, important information about the characters of the plants at various altitudes is needed, so that it is possible to extend its distribution through transplantation to other areas. Characterization can be performed based on morphological characters, chemical content, and protein banding patterns. This study aimed to describe the morphological characters, the chemical content (antioxidant capacity), and the pattern of protein bands by staining, using coomassie brilliant blue on C. pubescens in the Dieng Plateau. The research was conducted in the villages of Kejajar (1400 m asl), Patak Banteng (1900 m asl), and Sembungan (2400 m asl). The observations of morphological characters were conducted in the field and continued in the laboratory. Morphological characters, the chemical content (antioxidant capacity), and the banding pattern of protein of C. pubescens were analyzed descriptively. The results showed that the morphological characters of C. pubescens in Dieng Plateau varried in stems, leaves and fruits. The antioxidant capacity  decreased with decreasing habitat altitude, 2400 m asl altitude> 1900 m altitude> 1400 m asl. The Protein banding patterns did not vary, but the pattern in C. Papaya was different. The uniformity of the pattern of protein bands showed that genetic stability in C.pubescens was not affected by environmental factors.

Key words: Carica pubescens, morphological characters, antioxidant capacity, protein banding pattern.

Abstrak. Laily AN, Suranto, Sugiyarto. 2012. Karakterisasi Carica pubescens di Dataran Tinggi Dieng, Jawa Tengah berdasarkan sifat morfologi,  kapasitas  antioksidan,  dan  pola  pita  protein.  Nusantara  Bioscience  4:  16-21.  Carica  pubescens  Lenne  &  K.  Koch merupakan jenis tanaman buah yang pertamakali dibudidayakan di Amerika Selatan dan beradaptasi pada lingkungan dataran tinggi, misalnya Dataran Tinggi Dieng, Jawa Tengah (~2000 m dpl). C. pubescens memiliki daerah persebaran sempit dan variasi intraspesies terbatas atau belum diketahui. Oleh karenanya, diperlukan informasi mengenai karakter tanaman pada berbagai ketinggian sehingga dimungkinkan untuk memperluas daerah penyebaran melalui transplantasi di daerah lain. Karakterisasi dapat dilakukan berdasarkan karakter morfologi, kandungan kimia, dan pola pita protein. Penelitian ini bertujuan untuk mendeskripsikan karakter morfologi, kandungan kimia (kapasitas antioksidan), dan pola pita protein dengan pewarnaan coomassie brilliant blue pada C. pubescens di Dataran Tinggi Dieng. Penelitian lapangan dilakukan di Desa Kejajar (1400 m dpl), Patak Banteng (1900 m dpl), dan Sembungan (2400 m dpl). Pengamatan karakter morfologi dilakukan di lapangan dan dilanjutkan di laboratorium. Karakter morfologi, kandungan kimia (kapasitas antioksidan), dan pola pita protein C. pubescens  dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa karakter morfologi C. pubescens di Dataran Tinggi Dieng bervariasi pada batang, daun, dan buah. Kapasitas antioksidannya bervariasi dengan urutan dari ketinggian 2400  m dpl > 1900  m dpl > 1400  m dpl. Pola pita proteinnya tidak bervariasi antar ketinggian, namun berbeda dengan C. papaya. Keseragaman pola pita protein menunjukkan kestabilan genetik C. pubescens tidak dipengaruhi oleh perubahan lingkungan.

Kata kunci: Carica pubescens, karakter morfologi, kapasitas antioksidan, pola pita protein

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