Phytofabrication of silver nanoparticles by using aquatic plant Hydrilla verticilata

Neilesh Sable, Swapnil Gaikwad, Shital Bonde, Aniket Gade, Mahendra Rai

Abstract. Sable N, Gaikwad S, Bonde S, Gade A, Rai M. 2012. Phytofabrication of silver nanoparticles by using aquatic plant Hydrilla verticilata. Nusantara Bioscience 4: 45-49. In the context of current drive to developed new green technology in nanomaterials, synthesis of nanoparticles is of considerable importance. There has been considerable work done in the field of nanoscience and Nanotechnology during the last decade due to the introduction of various protocols for the synthesis of nanoparticles by using plants and microorganisms. Here we firstly report the extracellular phytosynthesis of silver nanoparticles (Ag-NPs) using aquatic plants Hydrilla verticilata. The characterization of the phytosynthesized Ag-NPs was done with the help of UV-Vis spectroscopy, FTIR, Nanoparticle Tracking Analysis (NTA), Zeta potential and SEM. The SEM micrograph revealed the synthesis of polydispersed spherical nanoparticles, with the average size of 65.55 nm. The phytofabricated Ag-NPs can be used in the field of medicine and agriculture, due to their antimicrobial potential.

Key words: phytofabrication, Hydrilla, Ag-NPs, SEM, FTIR

Abstrak. Sable N, Gaikwad S, Bonde S, Gade A, Rai M. 2012. Fitofabrikasi nanopartikel perak menggunakan tumbuhan akuatik Hydrilla verticilata. Nusantara Bioscience 4: 45-49. Dalam konteks mendorong pengembangan teknologi hijau yang baru pada nanomaterial, sintesis nanopartikel sangat penting. Selama dekade terakhir terjadi perkembangan yang cukup pesat dalam bidang nanosains dan nanoteknologi karena diperkenalkannya berbagai protokol untuk mensintesis nanopartikel menggunakan tumbuhan dan mikroorganisme. Dalam penelitian ini, dilaporkan fitosintesis ekstraseluler nanopartikel perak (Ag-NP) menggunakan tumbuhan akuatik Hydrilla verticilata untuk pertamakalinya. Karakterisasi Ag-NP yang difitosintesis dilakukan dengan bantuan spektroskopi UV-Vis, FTIR, Analisis Pelacakan Nanopartikel (NTA), potensial Zeta dan SEM. Mikrograf SEM menunjukkan hasil sintesis nanopartikel berbentuk bulat yang tersebar, dengan ukuran rata-rata 65,55 nm. Fitofabrikasi Ag-NP dapat dimanfaatkan dalam bidang kedokteran dan pertanian, karena memiliki potensi antimikroba.

Kata kunci: fitofabrikasi, Hydrilla, Ag-NPs, SEM, FTIR

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