In vitro rapid multiplication of Stevia rebaudiana: an important natural sweetener herb

SHIVAJI DESHMUKH, RAVINDRA ADE

Abstract. Deshmukh S, Ade R. 2012. In vitro rapid multiplication of Stevia rebaudiana: an important natural sweetener herb. Nusantara Bioscience 4: 105-108. Stevia rebaudiana Bertoni, belonging to family Asteraceae and natural sweet plant, but due to poor seed viability, fertility and vigor, Stevia cultivation is a challenging task. In the present study in vitro rapid multiplication method was established for S. rebaudiana by inoculating explants on M.S. medium, supplemented with different combination of phytoharmone. The maximum number of shoots (18.3±0.8) was obtained on M.S. medium supplemented with BAP + KIN (1.5 + 0.5 mg/L). The highest rooting percentage (95.25) was observed with (IAA 0.1 mg/L). The rooted plants were successfully established firstly in soil with coco peat (1:1) and then directly in ordinary soil.

Key words: Stevia rebaudiana, in vitro culture, multiplication, sweetener, micropropagation.

Abbreviations: IAA: Indole-3-acetic acid, BAP: 6-Benzyl amino purine, KIN: Kinetin, GA: Gibberellic acid. NAA: 1 Naphthalene acetic acid

 

Abstrak. Deshmukh S, Ade R. 2012. Perbanyakan cepat secara in vitro Stevia rebaudiana: herbal pemanis alami yang penting. Nusantara Bioscience 4: 105-108. Stevia rebaudiana Bertoni, anggota suku Asteraceae merupakan tanaman pemanis alami, namun karena viabilitas, kesuburan dan kekuatan benih yang buruk budidaya Stevia menjadi tugas yang menantang. Dalam penelitian ini metode perbanyakan cepat secara in vitro dilakukan pada S. rebaudiana dari eksplan inokulasi pada media MS, dilengkapi dengan kombinasi fitoharmon yang berbeda. Jumlah maksimum tunas (18,3±0,8) diperoleh pada media MS dengan BAP + KIN (1,5 + 0,5 mg/L). Persentase perakaran tertinggi (95,25) diamati dengan (IAA 0,1 mg/L). Tanaman berakar berhasil ditanam pertama kali pada tanah dengan coco peat (1:1) dan kemudian langsung di tanah biasa.

Kata kunci: Stevia rebaudiana, kultur in vitro, perbanyakan, pemanis, mikropropagasi.

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