Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus)

LINTAL MUNA, OKID PARAMA ASTIRIN, SUGIYARTO

Abstract. Muna L, Astirin OP, Sugiyarto. 2010. Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus). Nusantara Bioscience 2: 126-134. This experiment was performed to examine the effect of Pandanus conoideus Lam. var. yellow fruit extract on the percentage of the living foetus, the death intrauterus, heavy and long of foetus, foetus morphology, and skeleton structur of foetus. The experiment was used by 25 pregnant mice that randomly were divided into 5 groups, they contained 5 mice. Each group was given with the different dose. The P1 group (control) was given 1 mL sesame oil, the group P2, P3, P4 and P5 were respectively given the yellow fruit extract 0,02 mL, 0,04 mL, 0,08 mL and 0,16 mL. The P. conoideus var. yellow fruit extract was given orally on day 5 to 17 of gestation (organogenesis periode). Observation was carried out on day 18 of gestation by caesarean section to take the foetus from the uterus. Foetus morphology was observed after taking foetus from uterus, whereas observation of skeleton structure was made wholemount preparat with dual colourization, they are Alcian blue and Allizarin Red-S. The result was analyzed with one way anova. Results showed that giving yellow fruit extract didn’t influence to the percentage of the living foetus, the death intrauterus, heavy and long of foetus. The effect of giving yellow fruit extract to the maternal were abnormality skeleton (lordosis) of foetus in the dose 0.16 mL and obstacled to the ossification of foetus.
Key words: Pandanus conoideus var. yellow fruit, teratogenic, white rat.

Abstrak. Muna L, Astirin OP, Sugiyarto. 2010. Uji teratogenik ekstrak Pandanus conoideus varietas buah kuning terhadap perkembangan embrio tikus putih (Rattus norvegicus). Nusantara Bioscience 2: 126-134. Penelitian ini betujuan untuk mengkaji pengaruh pemberian ekstrak Pandanus conoideus Lam. var. buah kuning terhadap persentase fetus hidup, kematian intrauterus, berat dan panjang fetus, keadaan morfologi fetus, serta struktur skeleton fetus tikus putih. Dalam penelitian ini digunakan 25 tikus bunting yang dibagi menjadi lima kelompok secara acak, sehingga masing-masing kelompok terdiri dari lima ekor tikus. Setiap kelompok diberi dosis yang berbeda. P1 (kontrol) diberi 1 mL minyak wijen, P2, P3, P4 dan P5 diberi ekstrak masing-masing: 0,02 mL, 0,04 mL, 0,08 mL dan 0,16 mL. Ekstrak tersebut diberikan secara oral pada kebuntingan hari ke 5 sampai hari ke 17 (fase organogenesis). Pengamatan dilakukan pada hari ke 18 dengan cara bedah sesar untuk mengambil fetus dari uterus. Morfologi fetus diamati setelah fetus dikeluarkan dari uterus, sedangkan untuk pengamatan struktur skeleton dibuat preparat wholemount dengan pewarnaan ganda Alcian blue dan Allizarin Red-S. Hasil percobaan dianalisis dengan ANAVA satu jalur. Hasil penelitian menunjukkan bahwa pemberian ekstrak tidak berpengaruh terhadap persentase fetus hidup, kematian intrauterus, serta berat dan panjang fetus (P≥0,05). Pemberian ekstrak pada induk mengakibatkan kecacatan skeleton (lordosis) fetus pada dosis 0,16 mL dan menghambat osifikasi fetus.
Kata kunci: Pandanus conoideus var. buah kuning, teratogenik, tikus putih.

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