The effect of coconut water and naphthalene acetic acid (NAA) application on the in vitro growth of Paraphalaeonopsis serpentilingua from West Kalimantan

MUKARLINA, AGUSTINA LISTIAWATI, SRI MULYANI

 Abstract. Mukarlina, Listiawati A, Mulyani S. 2010. The effect of coconut water and naphthalene acetic acid (NAA) application on the in vitro growth of Paraphalaeonopsis serpentilingua from West Kalimantan. Nusantara Bioscience 2: 62-66. The ‘Ekor tikus’ orchid (Paraphalaenopsis serpentilingua J.J. Sm.) is an epidemic orchid in West Kalimantan. Now this orchid is facing a great conservation problem and threatened to endangered due to human exploitation. This research was conducted to find out the in vitro growth effect of P. serpentilingua by supplementation of NAA and coconut water in culture medium. The experiment was carried out using a Completely Randomized Design with two factors and five replicates. The result showed that supplemented NAA and coconut water on MS medium affected the emergence timing of buds, the average buds number, the average leaf number/buds, percentage of buds, the emergence timing of root, average root number and percentage of root. Medium that supplemented with 1.5 ppm NAA and 10% coconut water showed the fastest emergence timing of apical bud that is 13 days after planting. Medium supplemented with 0.5 ppm of NAA and 7.5% of coconut water shown the highest average number of bud was 11 buds.
Key words: Paraphalaenopsis serpentilingua, NAA, coconut water.

Abstrak. Mukarlina, Listiawati A, Mulyani S. 2010. Pengaruh aplikasi air kelapa dan asam naftalen asetat (NAA) pada pertumbuhan in vitro dari Paraphalaeonopsis serpentilingua dari Kalimantan Barat. Nusantara Bioscience 2: 62-66. Anggrek ‘ekor tikus’ (Paraphalaenopsis serpentilingua J.J. Sm.) adalah anggrek endemik di Kalimantan Barat. Sekarang anggrek ini menghadapi masalah konservasi yang serius dan terancam punah akibat eksploitasi manusia. Penelitian ini dilakukan untuk mengetahui pengaruh pertumbuhan P. serpentilingua oleh suplementasi NAA dan air kelapa secara in in vitro dalam medium kultur. Percobaan dilakukan menggunakan Rancangan Acak Lengkap dengan dua faktor dan lima ulangan. Hasil penelitian menunjukkan bahwa penambahan NAA dan air kelapa pada medium MS berpengaruh terhadap waktu munculnya tunas, rata-rata jumlah tunas, rata-rata jumlah daun / tunas, persentase kuncup, waktu munculnya akar, rata-rata jumlah akar dan persentase akar. Medium yang mengandung 1,5 ppm NAA dan 10% air kelapa menunjukkan waktu tercepat munculnya tunas apikal adalah 13 hari setelah tanam. Medium dengan penambahan 0,5 ppm NAA dan 7,5% air kelapa menunjukkan rata-rata tertinggi jumlah tunas adalah 11 kuncup.
Kata kunci: Paraphalaenopsis serpentilingua, NAA, air kelapa.

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