Biological screening of selected traditional medicinal plants species utilized by local people of Manokwari, West Papua Province

Obed Lense

Abstract. Lense O. 2011. Biological screening of selected traditional medicinal plants species utilized by local people of Manokwari, West Papua Province. Nusantara Bioscience 3: 145-150. The aim of the research was to determine the presence of alkaloids and anti-microbial activity in extracts from selected medicinal plants from Manokwari District, West Papua, Indonesia. The method of alkaloid testing followed the standard phytochemical methods. The procedure of the Calibrated Dichotomous Sensitivity (CDS) test was used for the antimicrobial bioassays. Results of biological screening suggested that all but one of the 56 species tested contained different levels of alkaloids. Eleven species showed anti-microbial activity using bioassays of responses to two bacteria, Salmonella typhi and Klebsiella pneumoniae, and two fungi Candida albicans, and Cryptococcus neoformans; none of the plant extracts showed an antimicrobial effect against the bacteria Escherichia coli. Extract of Planconella sp. was the most active one as it showed activity against three different organisms (C. albicans, C. neoformans, and S. typhi).

Key words: biological screening, local people, Manokwari, traditional medicinal plant, West Papua.

Abstrak. Lense O. 2011. Penapisan hayati beberapa jenis tumbuhan obat tradisional terpilih yang dimanfaatkan oleh masyarakat lokal Manokwari, Provinsi Papua Barat. Nusantara Bioscience 3: 145-150. Tujuan penelitian ini adalah untuk mengetahui adanya alkaloid dan aktivitas anti-mikroba ekstrak beberapa tanaman obat terpilih dari Kabupaten Manokwari, Papua Barat, Indonesia. Metode pengujian alkaloid mengikuti metode fitokimia standar. Prosedur uji Calibrated Dichotomous Sensitivity (CDS) digunakan untuk uji hayati anti-mikroba. Hasil penapisan hayati menunjukkan bahwa ke-56 jenis yang diuji mengandung alkaloid dengan kadar yang berbeda-beda, kecuali satu jenis. Sebelas jenis menunjukkan aktivitas anti-mikroba berdasarkan respons uji hayati terhadap dua bakteri, Salmonella typhi dan Klebsiella pneumoniae, dan dua jamur Candida albicans dan Cryptococcus neoformans, tidak satupun dari ekstrak tanaman yang menunjukkan efek anti-mikroba terhadap bakteri Escherichia coli. Ekstrak Planconella sp. adalah yang paling aktif karena menunjukkan aktivitas terhadap tiga organisme yang berbeda (C. albicans, C. neoformans, dan S. typhi).

Kata kunci: penapisan biologi, masyarakat lokal, Manokwari, tumbuhan obat tradisional, Papua Barat.

Download Full Text

This entry was posted in Jurnal. Bookmark the permalink.

Leave a Reply

Your email address will not be published. Required fields are marked *