Diversity of secondary metabolites from Genus Artocarpus (Moraceae)

ALIEFMAN HAKIM

Abstract. Hakim A. 2010. The diversity of secondary metabolites from Genus Artocarpus (Moraceae). Nusantara Bioscience 2:146-156. Several species of the Artocarpus genus (Moraceae) have been investigated their natural product. The secondary metabolites successfully being isolatad from Artocarpus genus consist of terpenoid, flavonoids, stilbenoid, arylbenzofuran, neolignan, and adduct Diels-Alder. Flavonoid group represent the compound which is the most found from Artocarpus plant. The flavonoids compound which are successfully isolated from Artocarpus plant consist of the varied frameworks like chalcone, flavanone, flavan-3-ol, simple flavone, prenylflavone, oxepinoflavone, Continue reading

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Hitherto unreported Agaricus species of Central India

ALKA KARWA, MAHENDRA KUMAR RAI

Abstract. Karwa A, Rai MK. 2010. Hitherto unreported Agaricus species of Central India. Nusantara Bioscience 2: 141-145. Melghat forest region from Central India was surveyed for occurrence of medicinal and culinary mushrooms during the years 2005-2008. Out of total 153 species, ten species of Agaricus were recorded from different localities. Of these, seven species namely Agaricus bitorquis, A. subrufescens, A. augustus, A. placomyces, A. essettei, A. basioanolosus and Agaricus sp. nov (a new species) are being reported for the first time from the region. The commercial button mushroom Agaricus bisporus lacks good breeding characters due to its bisporic nature. These wild cousins of the button mushroom Continue reading

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Histological study of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura larvae

YAYAN SANJAYA, DADANG MACHMUDIN, NANIN DIAH KURNIAWATI

Abstract. Sanjaya, Machmudin D, Kurniawati ND. 2010. Histological study of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura larvae. Nusantara Bioscience 2: 135-140. The effect of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura Fab. larvae has been carried out. The method was used Probit analysis, and based on LD 50 the virus was infected to know body weight and post infection damage.The damage of histological structure caused by SlNPV (0, 315, 390, 465, 540 dan 615 PIB/mL) was investigated after 0, 12, 24, 72 and 96 hours post infection. The histological material was prepared by using parafin method after fixation with Bouin Solution, then slice into 7 um and colored with Hematoxilin-Eosin. The result showed that the exposure SlNPV cause decreasing food consumption especially on Continue reading

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Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus)

LINTAL MUNA, OKID PARAMA ASTIRIN, SUGIYARTO

Abstract. Muna L, Astirin OP, Sugiyarto. 2010. Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus). Nusantara Bioscience 2: 126-134. This experiment was performed to examine the effect of Pandanus conoideus Lam. var. yellow fruit extract on the percentage of the living foetus, the death intrauterus, heavy and long of foetus, foetus morphology, and skeleton structur of foetus. The experiment was used by 25 pregnant mice that randomly were divided into 5 groups, they contained 5 mice. Each group was given with the different dose. The P1 group (control) was given 1 mL sesame oil, the group P2, P3, P4 and P5 were respectively given the yellow fruit extract 0,02 mL, 0,04 mL, 0,08 mL and 0,16 mL. The P. conoideus var. yellow fruit extract was given orally on day 5 to 17 of gestation (organogenesis periode). Continue reading

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Induced mutations by gamma ray irradiation to Argomulyo soybean (Glycine max) variety

DIANA SOFIA HANAFIAH, TRIKOESOEMANINGTYAS, SUDIRMAN YAHYA, DESTA WIRNAS

Abstract. Hanafiah DS, Trikoesoemaningtyas, Yahya S, Wirnas D. 2010. Induced mutations by gamma ray irradiation to Argomulyo soybean (Glycine max) variety. Nusantara Bioscience 2: 121-125. Induced mutation by gamma ray irradiation is one way to increase genetic variability of plants. This research used gamma ray irradiation on low doses (micro mutation). The aim of this research was to know the respons of doses level by micro mutation on gamma ray irridation to the growing and development of Argomulyo variety of soybean [Glycine max (L) Merr]. The seeds were irradiated by gamma ray micro mutation doses, namely 0 gray, 50 gray, 100 gray, 150 gray, and 200 gray. Variations that were obtained of each characters at generation M1 and M2 influences Continue reading

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Expression of vimetin protein and neurofilamen on forelimb buds of black-6 mice on gestation day 12 induced by 2-methoxyethanol by RTPCR

YULIA IRNIDAYANTI

Abstract. Irnidayanti Y. 2010. Expression of vimetin protein and neurofilamen on forelimb buds of black-6 mice on gestation day 12 induced by 2-methoxyethanol by Real Time RT-PCR. Nusantara Bioscience 2: 116-120. The aim of this study was to investigate impact
of 2-methoxyethanol, a major industrial chemical of plastic. Gene expression analysis is increasingly important in biological research, while real-time reverse transcription PCR (RT-PCR) is becoming the method of choice for high-through put and accurate expression
profiling of selected genes. Pregnant black-6 mice were injected intraperitoneally to 7.5 mmol/kg of 2- methoxyethanol on gestation day (GD) 10. Embryo were obtained on gestation day 12. Forelimb buds of embryo was collected and then put in the tube, which Continue reading

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Serum lipid profile and retinol in rats fed micronutrient rich edible vegetable oil blend

HAMID NAWAZ KHAN, HUMAIRA FAROOQI, SHAKIR ALI, JAFAR SALAMAT KHAN

Abstract. Khan HN, Farooqi H, Ali S, Khan JS. 2010. Serum lipid profile and retinol in rats fed micronutrient rich edible vegetable oil blend. Nusantara Bioscience 2: 109-116. The animal rats were given 10% oil mixed in fat free diet for one month or six months. In the experiment, the groups of rats were fed with the micronutrient (MN) rich blends mixed previously with 1% cholesterol, and their effects were tested on serum lipid profile. Most significant changes in the High Dencity Lipoprotein (HDL) cholesterol were observed in onemonth study where HDL increased from 24 mg/dl in group to 64 mg/dl in the Mustard palm olein oil blend (MP); in mustard oil (MO) alone fed rats, the HDL was 36 mg/dl. Serum retinol was analyzed as one of the important MN in rats receiving the diet mixed with the blend for various duration of time. The results assume great significance as MO or Continue reading

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Review: Biodiversity conservation strategy in a native perspective; case study of shifting cultivation at the Dayaks of Kalimantan

AHMAD DWI SETYAWAN

Abstract. Setyawan AD. 2010. Biodiversity conservation strategy in a native perspective; case study of shifting cultivation at the Dayaks of Kalimantan. Nusantara Bioscience 2: 97-108. Native tribes generally are original conservationists; they build genuine conservation strategy of natural resources and environment for sustainable living. Dayak is a native tribe of Kalimantan that has been living for thousands of years; they use shifting cultivation to manage the communal forest lands due to Kalimantan’s poor soil of minerals and nutrients, where the presence of phosphorus becomes a limiting factor for crops cultivation. In tropical forests, phosphorus mostly stored in the trees, so to remove it, the forest burning is carried out. Nutrients released into the soil can be used for upland rice (gogo) cultivation, until depleted; after that, cultivators need to open a forest, while the old land was abandoned (fallow) until it becomes forest again (for 20-25 years). The Continue reading

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Review: Colchicine, current advances and future prospects

RAVINDRA ADE, MAHENDRA KUMAR RAI

Abstract. Ade R, Rai MK. 2010. Colchicine, current advances and future prospects. Nusantara Bioscience 2: 90-96. Colchicine is a toxic natural compound and secondary metabolite commonly produced by plants like Colchicum autumnale and Gloriosa superba. It is originally used to treat rheumatic complaints, especially gout, and still finds its uses for these purposes today despite dosing issues concerning its toxicity. It is also prescribed for its cathartic and emetic effects. Initially oral colchicine has not been approved as a drug by U.S. Food and Drug Administration (FDA). But now FDA approved colchicine as a drug for some disorders. Colchicine’s present medicinal use is in the treatment of gout and familial mediterranean fever. It is also being investigated for its use as an anticancer drug. In neurons, axoplasmic transport is disrupted by colchicine. Due to all the pharmacological Continue reading

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The diet of spotted cuscus (Spilocuscus maculatus) in natural and captivity habitat

EVI W. SARAGIH, MARIA JUSTINA SADSOEITOEBOEN, FREDDY PATTISELANNO

Abstract. Saragih EW, Sadsoeitoeboen MJ, Pattiselanno F. 2010. The diet of spotted cuscus (Spilocuscus maculatus) in natural and captivity habitat. Nusantara Bioscience 2: 78-83. The ex-situ conservation of cuscus (Spilocuscus maculatus) under captivating condition is an alternative solution to protect cuscus from extinction. Diets became the main factor in order to support the domestication process. Particular studies on habitat and diet of cuscus have been carried out however there is still limited information on the nutrition  aspects of cuscus food. This study aimed to determine the diet type, palatability and nutrient in both natural habitat and captivating condition. The results indicated that there were 19 and 8 plant species identified as cuscus diets in both natural habitat and captivating condition. Continue reading

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